Z’ Factor including siRNA design quality parameter in RNAi screening experiments

siRNA and RNAi optimization.

The discovery and examination of the posttranscriptional gene regulatory mechanism known as RNA interference (RNAi) contributed to the identification of small interfering RNA (siRNA) and the comprehension of its enormous potential for clinical purposes. Theoretically, the ability of specific target gene downregulation makes the RNAi pathway an appealing solution for several diseases. Despite nu...

Experiments: Planning, Analysis, and Parameter Design Optimization

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RNAi Screening of the Tyrosine Kinome Identifies Therapeutic Targets in Acute Myeloid Leukemia Running Title: siRNA Screening in AML

Running Title: siRNA Screening in AML Jeffrey W. Tyner, Denise K. Walters, Stephanie G. Willis, Mary Luttropp, Jason Oost, Marc Loriaux, Heidi Erickson, Amie S. Corbin, Thomas O’Hare, Michael C. Heinrich, Michael W. Deininger, and Brian J. Druker 1 Division of Hematology and Medical Oncology, Oregon Health & Science University Cancer Institute, Portland, Oregon 97239, USA. 2 Howard Hughes Medic...

Platelet Activating Factor Receptor Activation Improves siRNA Uptake and RNAi Responses in Well-differentiated Airway Epithelia

Well-differentiated human airway epithelia present formidable barriers to efficient siRNA delivery. We previously reported that treatment of airway epithelia with specific small molecules improves oligonucleotide uptake and facilitates RNAi responses. Here, we exploited the platelet activating factor receptor (PAFR) pathway, utilized by specific bacteria to transcytose into epithelia, as a trig...

In-silico Approaches for RNAi Post-Transcriptional Gene Regulation: Optimizing siRNA Design and Selection